Mutagenesis:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Cell Culture:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Negative Control:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Recombinant:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Plasmid Preparation:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Clone Assay:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
Software:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
In Vivo:Article Title: Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion
Article Snippet: Cat# F530 Critical Commercial Assays Proteome Profiler cytokine array R&D Systems Cat# ARY028 Invisorb Spin Tissue Mini Kit Stratec Cat# 10321003 QuikChange Multi Site-Directed Mutagenesis Kit Agilent Cat # 200514 TruSight Tumor 15 Kit (TST15) illumina Cat# OP-101-1001 EnVisionTM Flex+, Mouse, high pH (Link) Dako Cat# K8002 EnVisionTM Flex+, Rabbit, high pH (Link) Dako Cat# K8009 EnVisionTM FLEX Target Retrieval Solution, low pH Dako Cat# K800521-2 Cell Culture Inserts, 8.0μm pore size Falcon Cat# 353097 Deposited Data Raw and analyzed data This paper NCBI BioProject ID PRJNA476326 Experimental Models: Cell Lines SW1463 ATCC Cat# CCL-234TM SW48 Kastrinakis et al., 1995 Summerhayes, Boston SW837 Sigma Cat# 91031104 HCT116 p53−/− Bunz et al., 1998 B. Vogelstein, Baltimore H1299 ATCC Cat# CRL-5803TM HT-29 DSMZ Cat# ACC 299 LS1034 ATCC Cat# CRL-2158 Colo-320 Sigma Cat# 93051113 SW1116 CLS Cat# 300348 DLD-1 DSMZ Cat# ACC 278 Experimental Models: Organisms/Strains Mouse: Apc1638N Fodde et al., 1994 N/A Mouse: p53null (−/−) (B6.129S2-Trp53/J) Jacks et al., 1994 or The Jackson Laboratory Strain# 002101 Mouse: floxR248Q (floxQ) Alexandrova et al., 2015 N/A Mouse: Rosa26CreERT2 (B6.129-Gt(ROSA)26Sor tm1(cre/ERT2)Tyj/J) The Jackson Laboratory Strain# 008463 Mouse: C57BL/6NCrl Charles River Strain# 027 Oligonucleotides Primers for QPCR and genotyping, see below this paper Table S1 siRNA TP53 Silencer® Select Ambion ID:s605 siRNA TP53 Silencer® Select Ambion ID:s607 siRNA PTPN11 Silencer® Select Ambion ID: s11526 siRNA PTPN11 Silencer® Select Ambion ID: s11525 siRNA PTPN11 Silencer® Select Ambion ID: s11524 siRNA Negative Control No. 2 (src2) Silencer® Select siRNA Ambion Cat# 4390847 Recombinant DNA pcDNA3 Invitrogen Replaced by pcDNA3.1 (+/−) pcDNA3-p53 R280K Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R283W Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R249S Mihara et al., 2003 PMID: 12667443 pcDNA3-p53 R175H Mihara et al., 2003 PMID: 12667443 pCMV-p53 R273H Gift by Bert Vogelstein Addgene plasmid # 16439 pCMV-p53 R248W Gift by Bert Vogelstein Addgene plasmid # 16437 pCMV-p53 R248Q This paper Cloned from pCMV-p53 R248W using a site-directed mutagenesis kit Software and Algorithms ImageJ software Open source https://imagej.net/Welcome PMID 22930834 GraphPadPRISM® Graphpad Software, Inc. https://www.graphpad.com/ Image LabTM Software Biorad http://www.bio-rad.com/de-de/product/image-lab-software Open in a separate window KEY RESOURCES TABLE GOF missense mutp53 proteins are stabilized by HSP90 and are often overexpressed in CRC but their contribution to in vivo cancer progression and possible therapeutic exploitability are unknown.
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